Fig 7.

Tulathromycin inhibits secretion of proinflammatory CXCL-8 in LPS-stimulated bovine macrophages in vitro. Levels of secreted CXCL-8 in macrophages treated with IMDM alone (control [ctrl]) or tulathromycin (1.0 mg/ml) for 6 h, 12 h, or 20 h at 37°C in the presence or absence of LPS (1 μg/ml). There were no detectable levels of CXCL-8 in unstimulated macrophages treated with tulathromycin (data not shown). Values are means ± SEM. n = 6 to 9/group. *, P < 0.05 versus control at each time point; #, P < 0.05 versus LPS-stimulated control at each time point.