Fig 2.

Combination treatment of rIFN-λ1 with a DAA reduces the emergence of drug-resistant replicon colonies. Huh-7 cells that stably maintain HCV subgenomic replicons representing either a WT GT1a (A) or an engineered 1a-NS3-R155K variant (B) were incubated with identical treatment regimens including one, two, or three HCV inhibitors at 1, 2.5, 5, 10, or 30 times the EC₅₀s estimated against the WT replicon. After a 25-day selection period in the presence of G418, surviving replicon colonies were fixed and stained with crystal violet. Results of a representative example of ≥2 independent experiments is shown.