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. 2013 Mar;57(3):1173–1179. doi: 10.1128/AAC.02047-12

Fig 1.

Fig 1

Linezolid resistance of strain 426-3147L correlates with expression of Cfr. (A) PCR analysis shows the presence of the cfr gene in 426-3147L. Linezolid-susceptible strains of S. aureus (RN4220) (S.a.) and of S. epidermidis (ATCC 12228) (S.e.) were used as negative controls. The S. sciuri strain carrying the pSCFS1 plasmid (S.s./pSCFS1) was used as a positive control. (B) Primer extension analysis of the extent of Cfr-mediated C8 methylation of A2503 in the 426-3147L strain. Controls were the following: S.e., cfr-negative S. epidermidis ATCC 12228; S.a., cfr-negative S. aureus; S.s./pSCFS1, cfr-positive S. sciuri carrying the pSCFS1 plasmid. The primer extension band corresponding to A2503 is indicated by an arrow. A weak reverse transcriptase stop in the cfr-negative control samples reflects C2 methylation of A2503 by an endogenous methyltransferase, RlmN (49). The bar graph below the gel shows the results of quantification of the band intensities.