Fig 2.

cfr gene in strain 426-3147L is located on a plasmid. (A) HindIII digest of total DNA or total plasmid preparation from 426-3147L. (B) The same gel after blotting and hybridization with the cfr-specific probe or probe for chromosomal gene se0011. (C) PCR detection of the cfr gene in the total plasmid preparation from 426-3147L or from S. aureus cells transformed with the plasmid prepared from 426-3147L. The plasmid vector pLI50 was used as a negative control. (D) HindIII digest of the total plasmid prepared from 426-3147L and from the transformed S. aureus cells. DNA bands attributed to the p7LC plasmid are indicated by arrowheads.