Fig 7.

Azole IC₅₀ determinations with CaCYP51 and Δ60HsCYP51. (A and B) IC₅₀s were determined with 1 μM CaCYP51 (A) and 0.4 μM Δ60HsCYP51 (B) for the medical azoles fluconazole (●), itraconazole (⊙), and ketoconazole (■). (C and D) IC₅₀s were also determined with 1 μM CaCYP51 (C) and 0.4 μM Δ60HsCYP51 (D) for the agricultural azoles epoxiconazole (●), prochloraz (■), propiconazole (▲), tebuconazole (⧫), triadimenol (○), prothioconazole (□), and prothioconazole-desthio (△) with itraconazole (⊙) as a control. Relative velocities of 1.00 correspond to actual velocities of 3.9 ± 0.3 min⁻¹ for CaCYP51 and 22.7 ± 4.8 min⁻¹ for Δ60HsCYP51.