Table 2.
Characteristics of C. jejuni mutants selected by Ery
| Lineagea and strainb | MIC (μg/ml) |
Mutation in 23S rRNA genec | Change in protein L4 | Change(s) in protein L22 | Mutation in cmeR | |
|---|---|---|---|---|---|---|
| Ery | Tyl | |||||
| Parent, NCTC 11168 | 1 | 4 | —d | — | — | |
| 68Ex-1 | ||||||
| 68E1-1 | 4 | 8 | — | — | A88E | — |
| 68E4-1 | 32 | 256 | — | — | A88E | Del490A |
| 68E8-1 | 64 | 128 | — | — | A88E | Del490A |
| 68E32-1 | 256 | 128 | — | G67V | A88E | Del490A |
| 68E64-1 | >512 | 256 | C2627A (3) | G67V | A88E | Del490A |
| 68Ex-2 | ||||||
| 68E1-2 | 4 | 8 | — | 59-G-60 | — | |
| 68E4-2 | 32 | 128 | — | 59-G-60 | G86E | — |
| 68E8-2 | 64 | 128 | — | 59-G-60 | A84D, G86E | — |
| 68E32-2 | 128 | 128 | — | 59-G-60 | A84D, G86V | — |
| 68E64-2 | 512 | 256 | — | 59-G-60 | A84D, G86V | — |
| 68Ex-268Ex-3 | ||||||
| 68E1-3 | 4 | 16 | — | R72I | — | — |
| 68E4-3 | 64 | 64 | — | R72I | V100L | H174N |
| 68E8-3 | 128 | 256 | — | R72I | 91-S-92 | H174N |
| 68E32-3 | >512 | >512 | — | R72I | — | H174N |
| 68E64-3 | >512 | >512 | A2074C (3) | R72I | — | H174N |
| Parent, 81176 | 1 | 4 | — | — | — | — |
| 68Ex-268Ex-376Ex-6 | ||||||
| 76E2-6 | 32 | 64 | — | — | — | — |
| 76E8-6 | 256 | >512 | A2074C (2) | — | — | — |
| 76E64-6 | >512 | >512 | A2074C (3) | — | — | — |
a
The lineages are named according to the parent strain and the antibiotic used for selection. 68 and 76 indicate that the parent strains are 11168 and 81-176, respectively, E represents selection by Ery, x depicts the various concentrations used for selection, and the last number indicates the lineage number.
b
The strains in the lineages are named similarly to the lineages, except that x is replaced by the actual antibiotic concentration from which the mutant was selected.
c
Each number in parentheses is the number of mutated copies of the 23S rRNA gene.
d
—, no mutations detected.