Table 2.
rdxA nonsense and frameshift mutationsa
| Strain | Concn (μg/ml) | Frameshift | Mutation description (base position)b | Mutation (codon, base position)c |
|---|---|---|---|---|
| Mtzr | ||||
| 71R | 32 | 0 | Substitution* (523), C→T or G→T | Stop codon (175, 523) |
| 83R | 32 | +1 | Insertion (576) | Stop codon (205, 613) |
| 93R | 32 | 0 | Substitution (415), C→T | Stop codon (139, 415) |
| 100R | 32 | 0 | Substitution* (523), C→T or G→T | Stop codon (175, 523) |
| 114R | 32 | 0 | Substitution (19), G→T | Stop codon (7, 19) |
| 115R | 32 | −1 | Deletion (496) | Stop codon (167, 499) |
| 121R | 16 | +2 | Insertion (6,7) | Stop codon (14, 40) |
| 123R | 32 | +1 | Insertion (23) | Stop codon (23, 67) |
| 205R | 32 | +1 | Insertion (595) | Stop codon (205, 613) |
| 239R | 8 | +1 | Insertion (313) | Stop codon (110, 328) |
| 244R | 32 | −7 | Deletion ×7 (179) | Stop codon (74, 220) |
| 249R | 32 | +5 | Insertion ×5 (178) | Stop codon (64, 190) |
| 263R | 32 | +1 | Insertion (193) | Stop codon (73, 217) |
| 269R | 32 | −1 | Deletion (191) | Stop codon (76, 226) |
| 270R | 32 | +1 | Insertion (193) | Stop codon (74, 220) |
| Mtzs | ||||
| JS114S | <8 | +4 | Insertion ×4 (300) | Stop codon (111, 331) |
| JS124S | <8 | +4 | Insertion ×4 (300) | Stop codon (111, 331) frameshift +4 |
| 101S | <8 | −1 | Deletion (86) | Stop codon (33, 97) |
a
Not listed in this table are other Mtzr strains that contained a 3-nucleotide deletion at codon 191, a 21-nucleotide (7-codon) deletion starting at codon 18, or the many missense mutations that resulted in amino acid differences in the encoded protein relative to rdxA of reference strain 26695. These amino acid replacements are shown in Fig. S1 in the supplemental material.
b
*, A total of 17 isolates have a “C” and 14 isolates have a “G” at position 523 (see Fig. S1 in the supplemental material).
c
The complete RdxA protein is 210 amino acids long.