Fig 4.

IC₅₀ determinations for azole antifungals. CYP51 reconstitution assays contained 2.5 μM CaCYP51. Voriconazole (filled circles) and prothioconazole-desthio (bullets) concentrations ranged from 0 to 4 μM, and prothioconazole (hollow circles) concentrations ranged from 0 to 100 μM, with the DMF concentration kept constant at 0.5% (vol/vol). Mean values from three replicates are shown along with associated standard error bars. Relative velocities of 1.0 equate to actual velocities of 0.080, 0.098, and 0.087 nmol min⁻¹ for the IC₅₀ determinations with voriconazole, prothioconazole, and prothioconazole-desthio, respectively.