Fig 5.

Q-Tof analysis of cell extracts of antifungal-treated Candida albicans. Solid-phase extraction of prothioconazole and prothioconazole-desthio standards contained peaks corresponding to [M+H]⁺ ions of m/z 344 and 312, respectively; [M+Na]⁺ ions were also observed at 366 and 334 (A and B). Cell extracts of C. albicans treated with prothioconazole contained both prothioconazole and prothioconazole-desthio (an example from cells grown in RPMI medium for 24 h at 37°C and 200 rpm is shown in panel C). RPMI and YPD media containing prothioconazole incubated at 37°C for 24 h contained both prothioconazole and prothioconazole-desthio (an example from RPMI medium is shown in panel D), and sterile deionized water containing prothioconazole incubated at 37°C for 24 h contained only prothioconazole (E). Each spectrum represents the sum of 10 scans.