. 2013 Mar;79(5):1436–1443. doi: 10.1128/AEM.03306-12

Table 2.

primers used in this study

Primer	Description	Sequence^a
Primer	Description	Forward	Reverse
Δdpr-F1	Upstream of dpr for Δdpr	`AAGCCTATCCACAGTGT`	`CAGTCGAGGATCCTCCTTAAAAAATATTCTT`
Δdpr-F2	Downstream of dpr for Δdpr	`GCTGACCTAGTAAAAGACAATCTGGATGTT`	`CATCTTCCAAAATATTGGT`
Δsod-F1	Upstream of sod for Δsod	`GTCTTCAGGGAGAAGATG`	`CAGTCGAGGATTCATTTCCTCTTTTCTTTT`
Δsod-F2	Downstream of sod for Δsod	`GCTGACCTAGTCTGTTGCTCGTCTTTATG`	`AACGAGCTATTCCATTCT`
ΔahpCF-F1	Upstream of ahpC for ΔahpCF	`GAATTGTGGAAAGTCGC`	`CAGTCGAGGATACACTTGTCCTCCTTCT`
ΔahpCF-F2	Downstream of ahpF for ΔahpCF	`GCTGACCTAGTGCTTTAGGTGCCTTTGA`	`TCGGAGTTCTCTCAACT`
ΔperR-F1	Upstream of perR for ΔperR	`TTGAGTGCTTGGTTGAAT`	`CAGTCGAGGATTTGACTCCTTCGTTATTC`
ΔperR-F2	Downstream of perR for ΔperR	`GCTGACCTAGTATTTGTCCAGACTGTCAA`	`GCCAATGTTCATGCTTTT`
perR-compl.-F1	perR complementation	`TGCGGATCCTTGATGATGCTAGGCAC`	`TCTTTGTTTCTTACCTCCTATTTCCCATAT`
Em^r	Erythromycin resistance gene for deletion	`ATCCTCGACTGGAAGCAAACTTAAGAGTG`	`ACTAGGTCAGCTTATTTCCTCCCGTTAAA`
Spc^r	Spectinomycin resistance gene for complementation, double deletion	`ATCCTCGACTGATCGATTTTCGTTCGTGA`	`ACTAGGTCAGCTTCCACCATTTTTTCAATTT`
ftf-N-terminal	Complementation	`ATCTCGAGTTTACTAAGTTCAACAATGG`	`AAGTCGACCCACCAATAACATTCCAAT`
ftf-C-terminal	Complementation	`AAGAAACAAAGAAAGCTCATCATGTTTCAAC`	`CGGCCGCGGTTCGTCTTGTTTCTCTCA`
ΔΔperR-F1	Upstream of perR for double deletion	`TTGAGTGCTTGGTTGAAT`^b	`CAGTCGAGGATTTGACTCCTTCGTTATTC`^b
ΔΔperR-F2	Downstream of perR for double deletion	`GCTGACCTAGTATTTGTCCAGACTGTCAA`^b	`GCCAATGTTCATGCTTTT`^b
dpr	Primers for quantitative PCR for dpr	`GTGGTTCAGGCTTCCTTTAT`	`ACTGTTTCTTCAAGTCTGGA`
sod	Primers for quantitative PCR for sod	`GTTTTGGCTCAGGTTGGGCT`	`ATAGTTTGGACGAACATTAC`
ahpC	Primers for quantitative PCR for ahpC	`TGGTTTAGCACAACGTGGAA`	`AGGGCAAACTTCTCCTGGAT`
sloR	Primers for quantitative PCR for sloR	`ACTGTCTCTGATGTGTTTGT`	`TCAACAAATATACTCCCATC`
gyrA	Primers for quantitative PCR for gyrA	`TCTCGCTGGACTTGTCACTG`	`CATCTAGGCGCATCACTTTG`

Restriction enzyme sequences are underlined.

Same primer as ΔperR-F1 or ΔperR-F2.