Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Source or reference |
|---|---|---|
| Strains | ||
| AL257 | Synechococcus elongatus PCC 7942 (wild type) | S. S. Golden |
| AL358 | Ptrc: glcP integrated at NSI | This work |
| AL360 | Ptrc: GLUT1 integrated at NSI | This work |
| AL361 | Ptrc: galP integrated at NSI | This work |
| AL434 | Ptrc: xylE-xylA-xylB integrated at NSI | This work |
| AL504 | Ptrc: gfp integrated at NSI | This work |
| AL505 | Ptrc: galP-gfp integrated at NSI | This work |
| AL535 | Same as AL361 but ΔglgC | This work |
| AL536 | Same as wild type but ΔglgC | This work |
| AL1030 | Ptrc: cscK-cscB integrated at NSI | This work |
| AL1067 | Ptrc: xylE integrated at NSI | This work |
| Plasmids | ||
| pAM2991 | NSI targeting vector; Ptrc | 20 |
| pBBR1MCS-5 | Gmr; broad-host-range vector | 21 |
| pSA69 | P15A ori; Ampr; PLlacO1::alsS-ilvC-ilvD | 19 |
| pAL18 | Same as pAM2991 but Ptrc: GLUT1 lacIq | This work |
| pAL40 | Same as pAM2991 but Ptrc: galP lacIq | This work |
| pAL46 | Same as pAM2991 but Ptrc: glcP lacIq | This work |
| pAL61 | Same as pAM2991 but Ptrc: gfp lacIq | This work |
| pAL63 | Same as pAM2991 but Ptrc: gfp-galP lacIq | This work |
| pAL65 | Same as pAM2991 but Ptrc: xylE lacIq | This work |
| pAL70 | Same as pAM2991 but Ptrc: xylE-xylA-xylB lacIq | This work |
| pAL82 | glgC knockout vector; Gmr | This work |
| pAL288 | Same as pAM2991 but Ptrc: cscK-cscB lacIq | This work |