Table 4.
Strain | OE | KO | Sugar | Growth rate (day−1) for time period (h), condition |
|||||||
---|---|---|---|---|---|---|---|---|---|---|---|
0–12, L | 12–24, D | 24–36, L | 36–48, D | 48–60, L | 60–72, D | 72–84, L | 84–96, D | ||||
AL257 | 0.082 ± 0.064 | ND | 0.110 ± 0.025 | ND | 0.161 ± 0.030 | ND | 0.163 ± 0.026 | ND | |||
AL257 | Glc | 0.128 ± 0.008 | ND | 0.238 ± 0.017 | ND | 0.204 ± 0.018 | ND | 0.469 ± 0.012 | ND | ||
AL536 | glgC | Glc | ND | ND | ND | ND | ND | ND | NA | NA | |
AL257 | Suc | 0.298 ± 0.010 | 0.081 ± 0.007 | 0.319 ± 0.015 | ND | 0.310 ± 0.017 | 0.087 ± 0.027 | 0.298 ± 0.025 | 0.071 ± 0.019 | ||
AL257 | Xyl | 0.149 ± 0.021 | 0.104 ± 0.017 | 0.285 ± 0.012 | ND | 0.350 ± 0.017 | 0.062 ± 0.012 | 0.446 ± 0.016 | 0.098 ± 0.016 | ||
AL361 | galP | 0.128 ± 0.017 | ND | 0.067 ± 0.010 | ND | 0.126 ± 0.018 | ND | 0.158 ± 0.004 | ND | ||
AL361 | galP | Glc | 0.992 ± 0.014 | 0.269 ± 0.083 | 0.428 ± 0.030 | 0.211 ± 0.015 | 0.540 ± 0.017 | 0.199 ± 0.012 | 0.285 ± 0.012 | 0.079 ± 0.021 | |
AL535 | galP | glgC | Glc | 0.438 ± 0.016 | 0.159 ± 0.013 | ND | ND | ND | ND | NA | NA |
AL358 | glcP | 0.122 ± 0.025 | ND | 0.118 ± 0.036 | ND | 0.112 ± 0.040 | ND | 0.279 ± 0.049 | ND | ||
AL358 | glcP | Glc | 0.730 ± 0.008 | ND | ND | ND | ND | ND | ND | ND | |
AL360 | GLUT1 | 0.140 ± 0.018 | ND | 0.112 ± 0.014 | ND | 0.115 ± 0.029 | ND | 0.292 ± 0.045 | ND | ||
AL360 | GLUT1 | Glc | 0.219 ± 0.029 | ND | 0.212 ± 0.027 | ND | 0.201 ± 0.051 | ND | 0.352 ± 0.055 | ND | |
AL1030 | cscKB | 0.134 ± 0.023 | ND | 0.106 ± 0.032 | ND | 0.141 ± 0.038 | ND | 0.148 ± 0.026 | ND | ||
AL1030 | cscKB | Suc | 0.327 ± 0.029 | 0.157 ± 0.021 | 0.401 ± 0.026 | 0.122 ± 0.034 | 0.376 ± 0.031 | 0.128 ± 0.011 | 0.412 ± 0.008 | 0.126 ± 0.009 | |
AL1067 | xylE | 0.102 ± 0.004 | ND | 0.141 ± 0.010 | ND | 0.161 ± 0.020 | ND | 0.172 ± 0.024 | ND | ||
AL1067 | xylE | Xyl | 0.140 ± 0.021 | ND | 0.179 ± 0.007 | ND | 0.154 ± 0.015 | ND | 0.192 ± 0.011 | ND | |
AL434 | xylEAB | ND | ND | 0.050 ± 0.011 | ND | 0.121 ± 0.011 | ND | 0.106 ± 0.014 | ND | ||
AL434 | xylEAB | Xyl | 0.395 ± 0.026 | 0.351 ± 0.014 | 0.572 ± 0.025 | 0.336 ± 0.012 | 0.471 ± 0.012 | 0.291 ± 0.020 | 0.313 ± 0.015 | 0.082 ± 0.020 |
These growth rates were calculated from the OD730 throughout diurnal conditions. All growth rates are the average rates for biological triplicates. The “±” signs indicate standard deviations of rates, where n = 3. Any growth rate calculated to be less than 0.050 day−1 is considered insignificant and is shown here as not detectable (ND). AL257 is the wild type. L, light condition; D, dark condition; OE, overexpression; KO, deletion; Xyl, xylose; Glc, glucose; Suc, sucrose; NA, not analyzed.