Fig 2.

Pooled bead sets tested with polyclonal detection agents or biotinylated enterotoxins. (A) Various concentrations of the indicated superantigens were added in a singleplex format to beads coupled with one of the six reagents (anti-SEB antibody [Ab], G5-8, anti-SEC antibody, L3, anti-TSST-1 antibody, or D10V). Biotinylated polyclonal antisuperantigen detection reagents were added, followed by SA-PE, and beads were assayed by flow cytometry (MFI values are shown). Reactions were run in duplicate, except for TSST. (B) In a multiplex format, biotinylated SEB and SEC were added directly to bead sets coupled with one of four capture agents, anti-SEB antibody, G5-8, anti-SEC antibody, or L3, followed by SA-PE. Beads were assayed by flow cytometry (MFI values are shown). Bead assays were evaluated in a single reaction.