Fig 2.

Phenotypic analysis of the rok mutants. Phenotypes of S. coelicolor M145 and its ΔSCO0794, ΔSCO1060, ΔSCO2846, ΔSCO6008 (Δrok7B7), ΔSCO6566, ΔSCO6600, and ΔSCO7543 mutants (designated GAM30 to GAM36, respectively) were tested on MM (A) and R2YE (B) microtiter plates supplemented with 1% (wt/vol) carbon sources as indicated. Cultures were grown for 120 h. (C) Complementation of the rok7B7 mutant GAM33 with pGAM15 harboring the rok7B7 gene and its native promoter. Phenotypes were analyzed on R2YE agar plates supplemented with 1% (wt/vol) mannitol, glucose, or xylose. Cultures were grown for 72 h. Note that development of the rok7B7 mutant is delayed on R2YE glucose and not completely blocked, and after 120 h normal sporulation occurred (see panel B).