Table 2.
Rok7B7 and carbon catabolite repression of agarase production
| Agar plate and supplement | Value for straina: |
Fold change | |||||
|---|---|---|---|---|---|---|---|
| M145 |
Δrok7B7 (GAM33) |
||||||
| Agarase (mm) | Total (mm) | Ratio | Agarase (mm) | Total (mm) | Ratio | ||
| MM | |||||||
| Agar | 6 | 26 | 0.23 | 6 | 26 | 0.23 | 1 |
| Mannitol | 5 | 25 | 0.2 | 5 | 25 | 0.2 | 1 |
| GlcN | 5 | 23 | 0.22 | 3 | 19 | 0.16 | 0.73 |
| Fructose | 6 | 26 | 0.23 | 6 | 25 | 0.24 | 1.04 |
| Maltose | 6 | 26 | 0.23 | 5 | 26 | 0.19 | 0.83 |
| GlcNAc | 5 | 26 | 0.19 | 5 | 26 | 0.19 | 1 |
| Glucose | 1 | 17 | 0.06 | 1 | 17 | 0.06 | 1 |
| Xylose | 5 | 25 | 0.2 | 0 | 15 | 0 | 0 |
| Chitin | 6 | 26 | 0.23 | 6 | 27 | 0.22 | 0.96 |
| Cellobiose | 1 | 17 | 0.06 | 1 | 17 | 0.06 | 1 |
| R2YE | |||||||
| Agar | 5 | 22 | 0.23 | 1 | 15 | 0.07 | 0.29 |
| Mannitol | 5 | 22 | 0.23 | 1.5 | 16 | 0.09 | 0.41 |
| GlcN | 5 | 22 | 0.23 | 1.5 | 16 | 0.09 | 0.41 |
| Fructose | 5 | 23 | 0.22 | 2 | 17 | 0.12 | 0.54 |
| Maltose | 5 | 22 | 0.23 | 3 | 20 | 0.15 | 0.66 |
| GlcNAc | 2 | 17 | 0.12 | 0 | 14 | 0 | 0 |
| Glucose | 1 | 18 | 0.06 | 1 | 18 | 0.06 | 1 |
| Xylose | 5 | 25 | 0.2 | 2 | 17 | 0.12 | 0.59 |
| Chitin | 5 | 22 | 0.23 | 2 | 17 | 0.12 | 0.52 |
| Cellobiose | 1 | 18 | 0.06 | 1 | 18 | 0.06 | 1 |
a
Agarase indicates agarase zone, measured from the outside of the colony to the edge of the zone of clearing. Total indicates the combined length of the agarase zone and the colony. Ratio indicates the length of the agarase zone to the combined length of the agarase zone and the colony. Fold change indicates the ratio of the expression level of Δrok7B7 to that of M145.