Fig 1.

LptE14 expression induces OM permeability to erythromycin antibiotics. Stationary-phase cultures of lptE diploid strains expressing chromosomally encoded lptE⁺ and either plptE⁺ (JY32), plptE14 (JY34), or plptE6 (JY17) (A) or cultures of ΔlptE2::kan strains carrying either plptE⁺ (JY33), plptE14 (JY35), or plptE6 (JY18) (B) were serially diluted and replica plated onto LB plates supplemented with ampicillin and Ara or plates additionally supplemented with 50 μg/ml Ery. Plates were incubated overnight at 37°C.