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LptE14 does not induce palmitoylation of lipid A by PagP. LPS was extracted from stationary-phase cultures of ΔlptE2::kan strains carrying plptE+ (JY33) and plptE14 (JY35), grown in LB medium supplemented with 0.2% arabinose, and radiolabeled with 32P. Samples were developed by TLC and then visualized by phosphorimaging. The ΔpldA ΔmlaC mutant (JCM537) was included as a positive control for hepta-acyl lipid A.
