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. 2013 Mar;195(6):1327–1334. doi: 10.1128/JB.02142-12

Fig 6.

Fig 6

Routing of LptD secretion via the SRP pathway restores levels of LptDOX and abolishes secA827::IS1 suppression. (A) Protein samples were obtained from stationary-phase cultures of ΔlptE ΔlptD::kan2 strains carrying pBAD33::lptE14 with plptD and either secA+ (MG1055) or secA827::IS1 (MG1056) or carrying pBAD33::lptE14 with psfmC-lptD and either secA+ (MG1059) or secA827::IS1 (MG1060). Samples were prepared under nonreducing conditions and immunoblotted with anti-LptD. (B) Stationary-phase cultures were serially diluted and plated onto LB plates with 0.2% arabinose or plates additionally supplemented with 50 μg/ml erythromycin. Plates were incubated overnight at 37°C. Cultures were of ΔlptE ΔlptD::kan2 strains carrying pBAD33::lptE+ and either plptD with secA+ (MG1053), plptD with secA827::IS1 (MG1054), psfmC-lptD with secA+ (MG1057), or psfmC-lptD (MG1058); alternatively, strains carried pBAD33::lptE14 and either plptD with secA+ (MG1055), plptD with secA827::IS1 (MG1056), psfmC-lptD with secA+ (MG1059), or psfmC-lptD (MG1060).