Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Escherichia coli strains | ||
| DH5α | F− endA1 thi-1 recA1 relA1 gyrA96 deoR nupG ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 hsdR17(rK− mK+) λ− | |
| MC4100 | F− araD139 Δ(arg-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 thi | 26 |
| NR754 | MC4100 Ara+ | 27 |
| JW3584 | BW25113 ΔsecB::kan | 28 |
| JY17 | NR754/plptE6 | 23 |
| JY18 | NR754 ΔlptE2::kan/plptE6 | 23 |
| JY32 | NR754/plptE+ | This study |
| JY33 | NR754 ΔlptE2::kan/plptE+ | This study |
| JY34 | NR754/plptE14 | This study |
| JY35 | NR754 ΔlptE2::kan/plptE14 | This study |
| JY105 | JY33 secA+ leuA::Tn10 | This study |
| JY106 | JY33 secA827::IS1 leuA::Tn10 | This study |
| JY107 | JY35 secA+ leuA::Tn10 | This study |
| JY108 | JY35 secA827::IS1 leuA::Tn10 | This study |
| MG934 | NR754 ΔlptE secB+/plptE+ | This study |
| MG935 | NR754 ΔlptE secB+/plptE14 | This study |
| MG949 | NR754 ΔlptE ΔsecB::kan/plptE+ | This study |
| MG950 | NR754 ΔlptE ΔsecB::kan/plptE14 | This study |
| MG1053 | NR754 ΔlptE ΔlptD::kan2 secA+ leuA::Tn10/pBAD33::lptE+/plptD | This study |
| MG1054 | NR754 ΔlptE ΔlptD::kan2 secA827::IS1 leuA::Tn10/pBAD33::lptE+/plptD | This study |
| MG1055 | NR754 ΔlptE ΔlptD::kan2 secA+ leuA::Tn10/pBAD33::lptE14/plptD | This study |
| MG1056 | NR754 ΔlptE ΔlptD::kan2 secA827::IS1 leuA::Tn10/pBAD33::lptE14/plptD | This study |
| MG1057 | NR754 ΔlptE ΔlptD::kan2 secA+ leuA::Tn10/pBAD33::lptE+/psfmC-lptD | This study |
| MG1058 | NR754 ΔlptE ΔlptD::kan2 secA827::IS1 leuA::Tn10/pBAD33::lptE+/psfmC-lptD | This study |
| MG1059 | NR754 ΔlptE ΔlptD::kan2 secA+ leuA::Tn10/pBAD33::lptE14/psfmC-lptD | This study |
| MG1060 | NR754 ΔlptE ΔlptD::kan2 secA827::IS1 leuA::Tn10/pBAD33::lptE14/psfmC-lptD | This study |
| JCM537 | MC4100 Arar/− ΔpldA ΔmlaC | 29 |
| Plasmids | ||
| plptE+ | lptE+ cloned into pBAD18 | 30 |
| pBAD33::lptE+ | lptE+ cloned into pBAD33 | This study |
| pBAD33::lptE14 | lptE14 cloned into pBAD33 | This study |
| plptD | lptD cloned into pET23/42 | 30 |
| psfmC-lptD | SfmC signal sequence fused to LptD mature sequence, cloned into pET23/42 | N. Ruiz |