Fig 3.
Analysis of eight clones of transconjugants obtained after filter-mating experiments between donor strain 515 (carrying ICE_515_tRNALys) and recipient strain S. pyogenes ATCC 12202. (A) Amplification of the right junction of the ICEs in these clones as for donor cells, using primers specific of the integrase genes and of the tRNALys gene (lane D). The negative control is S. pyogenes ATCC 12202, used as recipient cells (lane R). Lane M shows DNA molecular weight marker. (B) Analysis of the transconjugants by pulsed-field gel electrophoresis in parallel to the donor strain (lane D) and the recipient strain (lane R).