Fig 5.
Analysis of the site-specific excision of the CIME cat-ICE ery composite element integrated in the 3′ end of the tRNALys gene of transconjugants of S. agalactiae Nem316. (A) Schematic representation of the circular form (with its attI site) and the empty attB integration site resulting from excision of the composite element. Primers used to detect DNA molecules resulting from site-specific recombination events are indicated by letters. (B) Amplification of an attB site in 10 transconjugants of S. agalactiae Nem316. (C) Amplification of an attI site in the same 10 clones. ICE_515_tRNALys appears in black, and CIME_Nem_tRNALys appears in gray.