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Pulsed-field gel electrophoresis analysis of the two transconjugants (M1 and M2) obtained after conjugative mobilization in cis of the CIME cat by ICE ery. A strain deriving from Nem316 and harboring a tandem CIME cat–ICE ery (indicated by “D” on the schema) and a mutant strain resistant to rifampin and streptomycin deriving from Nem316 already harboring a resident CIME (indicated as recipient “R”) were used as donor and recipient strains, respectively.
