Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant phenotype or genotypea | Source or reference |
|---|---|---|
| Strains | ||
| S. agalactiae | ||
| 515 | Wild-type strain, carrying a putative ICE (ICE_515_tRNALys) | 19 |
| 2603V/R | Wild-type strain, carrying a composite ICE-IME element (ICE_2603_tRNALys), encoding a truncated conjugal transfer protein OrfD (ATPase VirB4 homolog) | 20 |
| COH1 | Wild-type strain, carrying a putative ICE (ICE_COH1_tRNALys), encoding a truncated conjugal transfer protein OrfD (ATPase VirB4 homolog) | 19 |
| 18RS21 | Wild-type strain, carrying a putative ICE (ICE_18RS21_tRNALys), encoding three truncated conjugal proteins (OrfC, OrfD, and OrfL) | 19 |
| Nem316 | Wild-type strain, carrying a putative CIME (CIME_Nem_tRNALys) | 21 |
| A909 | Wild-type strain, carrying a putative IME (IME_A909_tRNALys) | 19 |
| 515 (ICE_515_tRNALysery) | Strain carrying ICE_515_tRNALys tagged by an Eryr cassette, Eryr | This study |
| 515 (ICE_515_tRNALyscat) | Strain carrying ICE_515_tRNALys tagged by a Cmr cassette, Cmr | This study |
| 2603V/R (ICE_2603V/R_tRNALysery) | Strain carrying ICE_2603V/R_tRNALys tagged by an Eryr cassette, Eryr | This study |
| COH1 (ICE_COH1_tRNALysery) | Strain carrying ICE_COH1_tRNALys tagged by an Eryr cassette, Eryr | This study |
| 18RS21 (ICE_18RS21_tRNALysery) | Strain carrying ICE_18RS21_tRNALys tagged by an Eryr cassette, Eryr | This study |
| Nem316 (CIME_Nem_tRNALyscat) | Strain carrying carrying CIME_Nem_tRNALys tagged by a Cmr cassette, Cmr | This study |
| COH1 Rifr Strr | Spontaneous rifampin- and streptomycin-resistant mutant | This study |
| Nem316 Rifr Strr | Spontaneous rifampin- and streptomycin-resistant mutant | This study |
| A909 Rifr Strr | Spontaneous rifampin- and streptomycin-resistant mutant | This study |
| S. mutans | ||
| UA159 | Wild-type strain, no element integrated in the tRNALys CTT gene | 22 |
| UA159 Rifr Strr | Spontaneous rifampin- and streptomycin-resistant mutant | This study |
| S. salivarius | ||
| CIP102503 | Wild-type strain | Institut Pasteur collection |
| JIM8777 | Wild-type strain, with a putative IME integrated in the tRNALys CTT gene | 23 |
| CIP102503 Rifr Strr | Spontaneous rifampin- and streptomycin-resistant mutant | This study |
| JIM8777 Rifr Strr | Spontaneous rifampin- and streptomycin-resistant mutant | This study |
| S. thermophilus | ||
| LMG18311 | Wild-type strain, with a putative CIME-IME composite element integrated in the tRNALys CTT gene | 24 |
| LMG18311 Rifr Strr | Spontaneous rifampin- and streptomycin-resistant mutant | This study |
| S. uberis | ||
| 20388 | Wild-type strain | 25 |
| 21458 | Wild-type strain | 25 |
| 20388 Rifr Strr | Spontaneous rifampin- and streptomycin-resistant mutant | This study |
| 21458 Rifr Strr | Spontaneous rifampin- and streptomycin-resistant | This study |
| S. dysgalactiae | ||
| 14998 | Wild-type strain | 25 |
| 16192 | Wild-type strain | 25 |
| 14998 Rifr Strr | Spontaneous rifampin- and streptomycin-resistant | This study |
| 16192 Rifr Strr | Spontaneous rifampin- and streptomycin-resistant mutant | This study |
| S. pyogenes | ||
| ATCC 12202 | Wild-type strain | ATCC |
| ATCC 12202 Rifr | Spontaneous rifampin-resistant mutant | 26 |
| E. faecalis | ||
| JH2-2 | Rifr, Fusr | 27 |
| JH2-2 Rifr Strr | Spontaneous streptomycin-resistant mutant | This study |
| E. coli | ||
| EC101 | supE hsd-5 thi (lac-proAB) F (traD6 proAB lacIq lacZ M15) repA, derivative of strain TG1 | 18 |
| DH5α | supE44 lacU169 (ϕ80 lacZ M15) hsdR17 endA1 gyrA96 thi-1 relA1 | 28 |
| Plasmids | ||
| pG+host9 | 3.8 kb, pWV01-type thermosensitive replication origin (Ts) from pVE6002, Eryr | 29 |
| pSET4s | 3.8 kb, pWV01-type Ts from pVE6002, lacZ′, Spcr | 30 |
| pSET5s | 3.8 kb, pWV01-type Ts from pVE6002, lacZ′, Cmr | 30 |
| pSL1180 | 3.4 kb, pMB1 origin, superpolylinker for vector construction, Ampr | 31 |
a
Abbreviations: Ampr, ampicillin resistance; Cmr, chloramphenicol resistance; Eryr, erythromycin resistance; Fusr, fusidic acid resistance; Rifr, rifampin resistance; Spcr, spectinomycin resistance; Strr, streptomycin resistance.