Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics (genotype) | Reference |
|---|---|---|
| S.aureus strains | ||
| MW2 | Wild type; human clinical isolate (hlb::ϕSa3mw) | 21 |
| MW2ΔϕSa3mw | ϕSa3mw prophage deletion; strain obtained from MW2 (hlb+ ϕSa3mw−) | This study |
| MW2Δhlb | Beta-hemolysin gene null mutant; strain obtained from MW2 (Δhlb ϕSa3mw+) | This study |
| MW2ΔϕSa3mwΔhlb | Beta-hemolysin gene null mutant; strain obtained from MW2ΔϕSa3mw (Δhlb ϕSa3mw−) | This study |
| 8325-4 | Derivative of NCTC8325 cured of all known prophages (hlb+ ϕSa3mw−) | 43 |
| Plasmids | ||
| pKOR1 | S. aureus allele replacement vector | 24 |
| pKOR1-hlb1 | 2.0-kb PCR product for hlb null mutant in MW2, cloned into pKOR1 | This study |
| pKOR1-hlb2 | 2.0-kb PCR product for hlb null mutant in MW2ΔϕSa3, cloned into pKOR1 | This study |