. 2013 Mar;195(6):1194–1203. doi: 10.1128/JB.01786-12

Table 2.

Synthetic oligonucleotide primers

Function and plasmid or target	Primer designation	Sequence^a (5′–3′)	Nucleotide position^b
Function and plasmid or target	Primer designation	Sequence^a (5′–3′)	5′	3′
Plasmid construction
pKOR1-hlb1	attB1-hlb1-FW	`GGGGACAAGTTTGTACAAAAAAGCAGGCTGACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGA`	2087821	2087863
	attB1-hlb1-RV	`AGGTTCCCGCGGCCCCCAGTTTGGATACATAGAAACCTTGTAACAACAGTATT`	2088823	2088781
pKOR1-hlb1 or pKOR1-hlb2	attB2-hlb-FW	`GGGGACCACTTTGTACAAGAAAGCTGGGTACTCAAAGCTTACAATTTAACTTCTTAACTGAACCTAATTATGAT`	2089628	2089668
	attB2-hlb-RV	`AGGTTCCCGCGGTAGTGCTCAACTAACTAATAACTCGCTTCGTTCTAAAAGGT`	2090695	2090651
pKOR1-hlb2	attB1-hlb2-FW	`GGGGACCACTTTGTACAAGAAAGCTGGGTCTTTTAATATCACTTGAATTGATTAAATTCGCTGTGTAATACCTG`	2044905	2044943
	attB1-hlb2-RV	`AGGTTCCCGCGGAGTAAATGTTTTTTGAGTATAAAGATGCTGTAAATATAATTGAC`	2045942	2045899
Detection of excised chromosomal junction of each GI
ϕSa3mw	FW-ϕSa3mw	`TGATTTATCTAATGGCTTAGCTGC`	2045534	2045557
	RV-ϕSa3mw	`AGACACATAATGTGAAGTTTGTAAAAGTACTTAATGATA`	2088920	2088898
ϕSa2mw	FW-ϕSa2mw	`GCTTGTCCTTCATTTTCGTT`	1529034	1529054
	RV-ϕSa2mw	`GCAGGGTCTAGCTTTACCTTCT`	1575450	1575429
νSa3mw	FW-νSa3mw	`GATAAACGTCAAGCTTTGAAAGAAA`	838851	838875
	RV-νSa3mw	`GACGACCACAGTAGTTTAGATGAAG`	854252	854228
νSa4mw	FW-νSa4	`CGGGTTAAGTATGAACCTTACC`	2097110	2097132
	RV-νSa4	`CGAGTGGGTTAATATGTTAGAAGC`	2101110	2101087

Underlining indicates att sequences or SacII sites introduced into the primer.

The locations listed are specific for the chromosome of strain MW2 (GenBank accession no. AP004822 to AP004831).