Table 2.

Oligonucleotides used in this study

Nucleotide sequence (5′ to 3′)	Purpose	Source
ATGGCGCTCAAATTAGCCCCACATCTCACCTTG	dinB(C66A) mutagenesis forward primer	This work
ATTAAACTCGCGGTAGGGACAGCCGTATCG	dinB(C66A) mutagenesis reverse primer	This work
GCGCTCAAATTATGCGCACATCTCACCTTG	dinB(P67A) mutagenesis forward primer	This work
CGTATTAAACTCGCGGTAGGGACAGCC	dinB(P67A) mutagenesis reverse primer	This work
ATGGCGCTCAAATTAGCCGCACATCTCACCTTG	dinB(C66AP67A) mutagenesis forward primer	This work
ATTAAACTCGCGGTAGGGACAGCCGTATCG	dinB(C66AP67A) mutagenesis reverse primer	This work
CTGGTGCAAAAGCTGGATAAGCAGCAGGTGCTTTCGCAGCGAACGCGTTAATGAGCGATTGTGTTAGGCTGG	SOE-Lred primer 1; adds upstream chromosomal homology to cat	(32)
CTGGTAAAGTATACAGTGATTTCAGGGTTTGAGAAATGCGTAAAGATTCAGCATGCCATGGTCCATATGAATATCCTCC	SOE-Lred primer 2; adds dinB promoter homology to cat	(32)
ATGCTGAATCTTTACGCATTTCTCAAACCCTGAAATCACTGTATACTTTACCAGTGTTGAGAGGTGAGCAATGC	SOE-Lred primer 3; amplifies dinB with native promoter	(32)
GACCGATTTTTCAGCGAGAATTCGATGCATACAGTGATACCCTCATAATAATGCACACCAGAATATACATAATAGTATAC	SOE-Lred primer 4; amplifies dinB with downstream chromosomal homology	(32)
GCTCGTCAGACGATTTAGAGTCTGCAGTG	Undamaged control template for extension assay	This work
GCTCGTCAGACG/3-deaza-3-methylA/TTTAGAGTCTGCAGTG	Lesion-containing template for extension assay	This work
/HEX/CACTGCAGACTCTAAA	Fluorescently labeled primer for standing-start extension	This work