Fig 6.
Determination of Kd values for binding of PntR and PenR proteins to cognate DNA. Increasing concentrations of PntR and PenR were individually incubated with DNA fragments representing the one pnt intergenic region and the two pen intergenic binding regions, respectively. (A) Binding of PntR to the pntR-gapR intergenic region; (B) binding of PenR to the penR-gapN intergenic region; (C) binding of PenR to the penM-penH intergenic region. The error bars represent the means of three replicates. See Materials and Methods for details regarding data analysis and see Fig. S3 in the supplemental material for EMSA images.