(A) Deletion of the Y CTD inactivates holin function. Inductions in trans to λCamΔSR: no plasmid (○), pKT1-Y (●), pKT1-YΔC-term (□), and λ-Y lysogen harboring pKT1-YΔC-term (■). (B) LysA delays Y-mediated lysis. Inductions in trans to λ-Y: no plasmid (○) and plysA (●). Inductions in trans to λ-S105: no plasmid (□) and plysA (■). (C) YΔTMD1 delays Y-mediated lysis. Inductions in trans to λCamΔSR: no plasmid (●), pKT1-YΔTMD1 (▲), and pKT1-Y (■). Inductions in trans to λ-Y: no plasmid (○) and pKT1-YΔTMD1 (□).