Fig 6.
(A) Y, but not defective Y proteins, can be cross-linked into high-order oligomers. Photoactivated MBP cross-linking of whole-cell samples. Cells carrying either pKT2-Y (lanes 1 to 6) or pKT1-YL17P (lanes 7 to 12) were induced, labeled with MBP, and UV activated for the indicated lengths of time, in minutes, as described in Materials and Methods. (B) Lysis-defective Y proteins lose the ability to be cross-linked into high molecular weight (MW) species. Cultures carrying the indicated plasmids were induced, labeled with MBP, and UV-irradiated for 30 min (even-numbered lanes) or not (odd-numbered lanes). Lanes 1 and 2, Y; lanes 3 and 4, L9Q; lanes 5 and 6, L17P; lanes 7 and 8, A49T; lanes 9 and 10, G75S; lanes 11 and 12, K22M; lanes 13 and 14, L53P; lanes 15 and 16, G66D; monomer (asterisk), dimer (2 asterisks). Molecular weights are shown in thousands.