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Influence of enzyme concentration on enzyme activity. The FBP aldolase activities of LacD.1 and LacD.2 were measured at the enzyme concentrations 0.1, 0.325, 0.625, 1.25, 2.5, and 5 mM. Plotting of activity versus enzyme concentration revealed a linear relationship, with an R2 of 0.99 for both LacD.1 and LacD.2. See Materials and Methods for details.
