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. 2013 Mar;33(6):1254–1266. doi: 10.1128/MCB.01525-12

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Fig 7 — The Scl +40 element can enhance the Map17 promoter, but Map17 cannot rescue the Scl^Δ^40/^Δ⁴⁰ ES cell phenotype. (A) (Left) Schematic representation of constructs used for luciferase assays. (Right) Luciferase activity in BW5147, 416B, F4N, and MDCT cell lines. Data are normalized to Map17P-Luc. (B) Representative photographs of EBs generated from Scl^WT/WT, Scl^Δ^40/^Δ⁴⁰, and Scl^Δ^40/^Δ⁴⁰ mice overexpressing Flag-Map17 and pelleted EBs (right). Magnification, ×1.5 (top) or ×5 (bottom). (C) Histograms showing the percentages of TER119-positive (left) and cKit-positive (right) cells in day 8 EBs. (D) Graphs showing expression of Map17 (left) and Scl (right) during embryoid body differentiation. Data are normalized to the expression at day 8 in Scl^WT/WT EBs. WT, Scl^WT/WT; KO, Scl^Δ^40/^Δ⁴⁰.