Fig 3.

Generation of miR-7 expression vectors with or without QREs. (A) Schematic illustration of the hnRNPK minigene (pEGFP/hnRNPK). The putative QREs with their sequences are shown, as well as the location of miR-7. The mutated hnRNPK reporter gene is shown (pEGFP/hnRNPK:mQRE). (B) The pMIR-REPORT Luciferase (pLuc) and pMIR-REPORT Luciferase harboring sequences from the 3′-UTR of the EGF receptor with the miR-7 targeting sequences (pLuc:EGFR3′-UTR) are depicted. HEK293 cells were cotransfected with pLuc or pLuc:EGFR3′-UTR, along with increasing amounts of either the wild-type hnRNPK minigene (pEGFP/hnRNPK) or the mutated minigene (pEGFP/hnRNPK:mQRE), and assayed for luciferase activity normalized to that for Renilla.