Fig 6.

The QKI isoforms reduce pri-miR-7-1 processing by Drosha. (A) Cell lysates prepared from U343 cells transfected with siCTL, siQKI, or siDrosha and immunoblotted with anti-pan-QKI, anti-Drosha, or antitubulin antibodies. The migration of the molecular mass markers is shown on the left. (B) Cell lysates prepared from U343 cells transfected with siCTL, siQKI, or siDrosha were immunoprecipitated with anti-Drosha or IgG antibodies. The bound RNA was purified, and pri-miR-7-1 was detected by real-time qRT-PCR. (C) Fluorescence in situ hybridization performed using antisense pri-miR-7-1 as a probe on siCTL- or siQKI-transduced U343 cells. The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI), and the images were merged.