Fig 4.

A fragment of the SNF2 domain of p400 binds directly to recombinant TIP60. (A) Schematic representing different domains of p400. The indicated fragments of p400 were expressed as GST-tagged proteins. (B and C) p400 fragment(949-1372) interacts with rTIP60. The indicated GST proteins were incubated with rTIP60 in bacterial lysate for an hour at 4°C. The glutathione-agarose-bound proteins were immunoblotted with the indicated antibodies. Five percent of input is shown for each sample. The asterisk in the second lane of panel C indicates the size of the p400(476-948) fragment. (D) GST pulldown similar to that for panel B using p400(949-1372) or p400(2193-2587).