Fig 5.

rTIP60's activity is inhibited by p400(949-1372). (A) Estimation of purified p400 fragments. The indicated amounts of purified p400 fragments [p400(1-475) or p400(949-1372)] were resolved and immunoblotted with anti-GST antibody. (B) p400(949-1372) inhibits rTIP60's HAT activity. An ELISA-based HAT assay was performed with rTIP60 in the presence of 100 ng of GST, GST-p400(1-475), or GST-p400(949-1372). The HAT activity is normalized to that seen in the presence of GST alone. Means ± SD from three independent experiments are shown.