Fig 4.

FOSL1 downregulation increases integrin expression on the cell surface and cell adhesion. (A) HUVEC silenced for FOSL1 (shFOSL1) exhibited increased numbers of focal adhesion plaques containing αvβ3. Cell staining of integrins αv and β3 in HUVEC silenced for FOSL1 and in FOSL1-silenced cells infected with a viral vector expressing an shRNA-resistant FOSL1 mutant (rescue) is shown. Scale bar, 10 μm. DAPI (4′,6′-diamidino-2-phenylindole) was used for nuclear staining. (B) HUVEC adhesion to vitronectin-coated filters (original magnification, ×40). (C) Quantification of cell adhesion measured by crystal violet staining. Data are presented as OD values of independent experiments ± SD (n = 3).