Fig 5.

Rqh1 is not required for chromosome fusion when Pot1 expression is reduced. (A) NotI-digested S. pombe chromosomal DNA from the wild-type, pot1Δ, nmt-pot1-aid, and nmt-pot1-aid rqh1Δ strains (JY746, KTA037, NH001, and NH002) was analyzed using PFGE. Probes specific for the telomeric NotI fragments (I, L, and M) were used (66). Cells were preincubated in the presence of thiamine (15 μg/ml) for 24 h to reduce the expression of pot1 (0 h) at 30°C. Then, both thiamine and auxin (0.5 mM 1-naphthaleneacetic acid) were added and incubated for 12 h and 48 h. (B) The telomere lengths of the wild-type, nmt-pot1-aid, and nmt-pot1-aid rqh1Δ strains were analyzed using Southern hybridization at 30°C. Cells were incubated as described for panel A.