Fig 6.

Top3 is required for viability of pot1Δ when Rad51 is active. (A) Spotting assay of 10-fold serial dilutions of cells. pot1Δ rqh1-hd, pot1Δ rqh1-hd top3Δ, and pot1Δ rqh1-hd top3Δ rad51Δ cells expressing Pot1 from a plasmid (GT000, KTA028, and KTA029) were plated on EMM+AL and YEA+FOA at 25°C. The Pot1 plasmid is retained on EMM+AL, and cells that lost the plasmid were selected for on YEA+FOA at 25°C. (B) NotI-digested S. pombe chromosomal DNA from the wild type, two independent pot1Δ rqh1-hd top3Δ rad51Δ isolates, and one pot1Δ rqh1Δ rad51Δ isolate (JY746, KTA039, KTA040, and TN042) was analyzed using PFGE at 25°C. (C) The telomere lengths of the same strains were analyzed using Southern hybridization at 25°C.