Fig 7.

CARMA1-dependent BCL10 degradation in B cells does not require cIAP1/2 expression. WT DT40 cells (A) or Ramos cells expressing myc-CARMA1-ΔPRD (B) were pretreated with the cIAP1/2 antagonist BV6 or vehicle (DMSO) for 1 h at 37°C and were incubated with P/I and CHX (A) or just CHX (B) for the indicated times. WCLs were immunoblotted with the antibodies indicated on the left. Signal intensities of BCL10 or CARMA1 relative to ERK (A) or actin (B) are shown beneath the blots (zero time points = 1).