Fig 8.

TAK1 overexpression downregulates CARMA1-dependent NF-κB activity and reduces BCL10 levels. (A) 293T cells were transfected for 48 h with Igκ2-IFN-luciferase (NF-κB reporter) and pRL-TK (transfection control) and combinations of empty vector (−) or the indicated expression vectors (labels at the bottom of the graphs). Luciferase activity was determined as described in Materials and Methods. Bottom, WCLs were analyzed by immunoblotting for the proteins indicated to the left or right of the blots. (B) 293T cells were cotransfected for 48 h with empty vector (−) or the indicated expression vectors (top of blots) and were immunoblotted for BCL10, CARMA1, and TAK1 (left). Quantifications, carried out as described in Materials and Methods, are shown below the BCL10 blots. **, P > 0.01; *, P > 0.05; ns, not significant, unpaired Student's t test.