Fig 5.

Depletion of the DDB1-CUL4 complex results in a phenocopy of UCH-L1 expression. (A and C) HEK-293T cells were transduced with lentiviruses carrying the indicated shRNAs for 5 days, and extracts were immunoblotted using the indicated antibodies. (B) HEK-293T transduced to carry a doxycycline-regulated shRNA targeting DDB1 were incubated with or without doxycycline for 5 days, followed by immunoprecipitation and immunoblotting with the indicated antibodies. (D) HEK-293T cells were transduced with the indicated constructs, followed by immunoblotting for the indicated antibodies. shNS and NS, nonsilencing shRNA.