Fig 7.
UCH-L1 reduces ubiquitination of raptor. (A and C) HEK-293T cells were transiently transfected (+) or not transfected (−) with HAUbG76A, and extracts (prepared with 1% SDS) were subjected to immunoprecipitation and immunoblotting with the indicated antibodies. Lanes separated by dashed lines were from different immunoblots. (B) HEK-293T cells were transduced with the indicated constructs, and extracts (prepared with CHAPS) were subjected to immunoprecipitation and immunoblotting (IB) with the indicated antibodies.