Table 4.
Validity values of the universal 16S rRNA gene PCR for the detection of venous access port-related bloodstream infectiona
| Test | No. of cases/total (%) (95% confidence interval) |
|||
|---|---|---|---|---|
| S | SP | PPV | NPV | |
| PCR with any sample | 12/15 (80.0) (51.9–95.7) | 151/204 (74.0) (67.8–80.3) | 12/65 (18.5) (8.3–28.7) | 151/154 (98.1) (94.4–99.6) |
| PCR with CABS | 8/15 (53.3) (26.6–78.7) | 175/204 (85.8) (80.7–90.8) | 8/37 (21.6) (7.0–36.2) | 175/182 (96.2) (93.1–99.2) |
| PCR with CAAS | 9/15 (60.0) (32.3–83.7) | 171/204 (83.8) (78.5–89.1) | 9/42 (21.4) (7.8–35.0) | 171/177 (96.6) (93.7–99.6) |
| PCR with PSF | 7/15 (46.7) (21.3–73.4) | 177/204 (86.8) (81.9–91.7) | 7/34 (20.6) (5.5–35.7) | 177/185 (95.7) (92.5–98.9) |
| PCR with ISB | 11/15 (73.3) (44.9–92.2) | 178/204 (87.3) (82.4–92.1) | 11/37 (29.7) (13.7–45.8) | 178/182 (97.8) (94.5–99.4) |
a
S, sensitivity; SP, specificity; PPV, positive predictive value; NPV, negative predictive value; CABS, port content aspirate before sonication; CAAS, port content aspirate after sonication; PSF, port sonication fluid; ISB, port internal surface biofilm.