Table 1.
Data for the 39 stool specimens containing Shiga toxin genes
| Stool specimen | Clinical symptoma | Results by standard procedureb |
Result with the following chromogenic medium: |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Presence of the following virulence factor: |
STEC isolated | CHROMagar STECc | CHROMagar STEC O104d | ||||||
| stx1 | stx2 | eae | hlyA | aggR | |||||
| 2011/04475 | — | − | + | + | + | None | (+) | − | |
| 2011/04522 | HUS | − | + | − | − | + | O104:H4 | + | + |
| 2011/04623 | BD | − | + | − | − | + | O104:H4 | + | + |
| 2011/04632 | — | − | + | − | − | + | O104:H4 | + | + |
| 2011/04709 | Diarrhea | − | + | + | + | − | O157:H7 | + | − |
| 2011/04786 2011/04786 | — | + | + | + | + | − | O26:F11 | + | − |
| 2011/04875 | BD HUS | − | + | + | + | None | −‡ | − | |
| 2011/04989 | Abdominal cramps | − | + | − | − | + | O104:H4 | + | + |
| 2011/05004 | BD | − | + | − | − | + | O104:H4 | + | − |
| 2011/05008 | BD | + | + | + | + | O157:[F7] | + | − | |
| 2011/05017 | BD, ulcerative colitis | + | + | + | + | O118:F16 | + | − | |
| 2011/05049 | — | + | − | + | + | O26:[F11] | + | − | |
| 2011/05050 | BD | + | − | + | + | O103:F2 | + | − | |
| 2011/05102 | HUS | − | + | − | − | − | Rnew2:F14 | +‡ | − |
| 2011/05105 | BD | − | + | + | + | R121:F19 | + | − | |
| 2011/05132 2011/05132 | — | − | + | − | − | − | R148:F8 | + | − |
| 2011/05602 | — | − | + | − | − | + | O104:H4 | + | + |
| 2011/05638 | HUS | + | + | + | + | O26:F11, O157:H7 | + | − | |
| 2011/06543 | BD, HUS | − | + | − | − | − | None | (+) | − |
| 2011/06667 | BD, HUS | + | − | + | + | O103:[F2] | + | − | |
| 2011/06833 | — | − | + | − | − | − | R113:F21 | +‡ | − |
| 2011/08768 | — | − | + | + | + | O26:[F11] | + | − | |
| 2011/09110 | — | − | + | − | − | + | O104:H4 | + | − |
| 2011/09378 | HUS | − | + | − | − | − | O91:F10 | +‡ | − |
| 2011/10085 | BD | + | + | + | + | O157:H7 | + | − | |
| 2011/10600 2011/10600 | — | + | + | + | + | Rnew1:F2 | + | − | |
| 2011/11333 | — | − | + | − | + | − | R171:F25 | +‡ | − |
| 2011/11527 | Diarrhea, HUS | − | + | + | + | R80:F2 | −‡ | − | |
| 2011/11551 | Diarrhea, HUS | − | + | − | − | − | R174:F21 | +‡ | − |
| 2011/11802 | HUS | − | + | + | + | Rnew3:[F2] | +‡ | − | |
| 2012/02395 | — | + | + | − | + | − | Rnew4:[F19] | −‡ | − |
| 2012/02396 | None | + | + | − | + | − | None | (+) | − |
| 2012/02846 | BD | + | + | + | + | O157:H7 | + | − | |
| 2012/02998 | — | − | + | − | − | − | R148:F8 | −‡ | − |
| 2012/03065 | — | + | − | + | + | O26:F11 | + | − | |
| 2012/03794 | Diarrhea, HUS | − | + | + | + | O157:[F7] | + | − | |
| 2012/03796 | — | + | − | + | + | O26:F11 | + | − | |
| 2012/05675 | Diarrhea | + | + | + | + | O157:[F7] | + | − | |
| 2012/06881 | Diarrhea, HUS | − | + | + | + | O157:[F7] | + | − | |
BD, bloody diarrhea; HUS, hemolytic-uremic syndrome; —, no information.
The virulence gene content reported is that obtained both for stool specimens and from isolated colonies, except for the four cases with no isolated STEC strain; hlyA refers to EHEC hlyA; the O type determined by rfb-RFLP and the H type determined by fliC sequencing are denoted R and F, respectively; an F type in brackets indicates the presence of nonmotile strains; R types not present in our rfb-RFLP database are denoted Rnew1 to Rnew4.
+ and +‡, mauve colonies with the same STEC isolated as that isolated by the standard procedure and obtained following enrichment protocols 1 and 2, respectively; (+), no virulence genes were detected in mauve colonies; −‡, no mauve colonies were detected following either enrichment protocol 1 or enrichment protocol 2.
+, mauve colonies with the same STEC isolated as that isolated by the standard procedure and obtained following enrichment protocol 1; −, no mauve colonies following enrichment protocol 1.