Table 2.
Data for the 15 Shiga toxin-negative, eae-positive stool specimens
| Stool specimen | Clinical symptomsa | Results by standard procedureb |
Result with the following chromogenic medium: |
||||
|---|---|---|---|---|---|---|---|
| Presence of the following virulence factor: |
E. coli serotype isolated | CHROMagar STECc | CHROMagar STEC O104d | ||||
| eae | hlyA | bfpA | |||||
| 2011/04625 | BD | + | + | − | O145:F28 | + | − |
| 2011/05678 | — | + | − | − | O111:F21 | −‡ | − |
| 2011/07736 | — | + | − | − | R38:[F9] | + | − |
| 2011/07971 | BD | + | − | − | R101/162:F33 | + | − |
| 2011/08324 | — | + | − | − | Rnew5:F49 | −‡ | − |
| 2011/08954 | BD | + | + | − | O145:F28 | + | − |
| 2011/09320 | Diarrhea | + | − | − | Rnew6:[F21] | + | − |
| 2011/09604 | — | + | − | − | None | (+) | − |
| 2011/09777 | — | + | + | − | O26:F11 | + | − |
| 2011/10026 | BD | + | − | − | None | −‡ | − |
| 2012/00278 | BD | + | − | − | None | −‡ | − |
| 2012/00781 | Diarrhea | + | − | − | Noned | +‡e | − |
| 2012/00872 | HUS | + | − | − | R76:F7 | + | + |
| 2012/06703 | Diarrhea | + | + | − | R121:F19 | + | − |
| 2012/08776 | — | + | − | − | None | − | − |
BD, bloody diarrhea; HUS, hemolytic-uremic syndrome; —, no information.
The virulence gene content reported is that both for stool specimens and for isolated colonies, except for the four cases with no isolated eae-positive E. coli strain; the O type determined by rfb-RFLP and the H type determined by fliC sequencing are denoted R and F, respectively; an F type in brackets indicates the presence of nonmotile strains; R types not present in our rfb-RFLP database are denoted Rnew5 and Rnew6.
+ and +‡, mauve colonies with the same STEC isolated as that isolated by the standard procedure and obtained following enrichment protocols 1 and 2, respectively; (+), no virulence genes were detected in mauve colonies; − and −‡, no mauve colonies following enrichment protocols 1 and 2, respectively.
+, mauve colonies with the same STEC isolated as that isolated by the standard procedure and obtained following enrichment protocol 1; −, no mauve colonies following enrichment protocol 1.
An eae-positive R51:F40 strain was recovered only on CHROMagar STEC.