Table 1.
Species/sequence variant | Pyrosequencing signature sequenceb | Identityc | Percentaged |
---|---|---|---|
Candida albicans 1 | GTCAA AGTTT GAAGA TATAC GTGGT GGACG TTACC | 35 | 65.3 |
Candida albicans 2 | GTCAA AGTTT GAAGA TATAC GTGGT aGACG TTACC | 34 | 24.9 |
Candida albicans 3 | GTCAA AGTTT GAAGA TATAC GTGGT aGACG cTACC | 33 | 7.0 |
Candida albicans 4 | GTCAA AGTTT GAAGA TATAC GTGGT aGACG TTgCC | 33 | 2.8 |
Candida stellatoidea (CBS1905) | GTCAA AGTTT GAAGA TATAC GTGGT GGACG TTACC | 35 | ND |
Candida africana | GTCAA AGTTT GAAGA TATAC GTaGT aGACG TTACC | 33 | NA |
Candida dubliniensis 1 | GTCAA AGTTT GAAGA ataAa aTGGg cGACG ccAga | 24 | 88.0 |
Candida dubliniensis 2 | GTCAA AGTTT GAAGA ataAa aTGGc –GACG ccAga | 24 | 12.0 |
Sequences are aligned against the 35-bp signature of the most common C. albicans variant (C. albicans 1). A total of 213 isolates (C. albicans), 25 isolates (C. dubliniensis), and 15 isolates (C. africana) were subjected to pyrosequencing.
Bold type indicates totally conserved nucleotides. Lowercase letters indicate positions in which the sequence differs from that of C. albicans 1.
Identity is given as the number of identical bases compared to the signature sequence of C. albicans 1.
Percentage of isolates of a given species with that particular signature sequence. ND, not determined; NA, not applicable.