Table 1.

Comparison of single-target assay with multiplex RT-qPCR assay

Virus	Dilution	Cq
		Single-target RT-qPCR	Multiplex RT-qPCR
			PRRSV	PCV-2	CSFV/ASFV/SuHV-1/FMDV	Beta-actin
PRRSV EU	1.00E−02	26.3	25.9	No Cq	No Cq	28.9
	1.00E−03	29.9	28.9	No Cq	No Cq	28.8
	1.00E−04	33.0	31.8	No Cq	No Cq	29.1
	1.00E−05	36.6	35.4	No Cq	No Cq	28.8
	1.00E−06	38.3	41.0a	No Cq	No Cq	28.8
	1.00E−07	No Cq	No Cq	No Cq	No Cq	28.7
	1.00E−08	No Cq	No Cq	No Cq	No Cq	29.0
PRRSV NA	1.00E−02	21.2	21.9	No Cq	No Cq	27.7
	1.00E−03	24.8	25.6	No Cq	No Cq	28.6
	1.00E−04	27.5	28.7	No Cq	No Cq	28.7
	1.00E−05	31.1	34.3	No Cq	No Cq	28.5
	1.00E−06	34.3	No Cq	No Cq	No Cq	28.5
	1.00E−07	37.0	No Cq	No Cq	No Cq	28.9
	1.00E−08	No Cq	No Cq	No Cq	No Cq	29.0
PCV2	1.00E−02	19.2	No Cq	19.0	No Cq	28.7
	1.00E−03	22.3	No Cq	22.2	No Cq	28.5
	1.00E−04	26.1	No Cq	25.9	No Cq	29.0
	1.00E−05	28.9	No Cq	28.6	No Cq	28.8
	1.00E−06	31.3	No Cq	31.5	No Cq	29.0
	1.00E−07	35.7	No Cq	37.9	No Cq	29.1
	1.00E−08	No Cq	No Cq	No Cq	No Cq	29.2
CSFV	1.00E−03	22.1	No Cq	No Cq	23.3	28.7
	1.00E−04	26.0	No Cq	No Cq	26.2	28.9
	1.00E−05	29.8	No Cq	No Cq	30.1	29.0
	1.00E−06	33.1	No Cq	No Cq	33.9	28.9
	1.00E−07	36.6	No Cq	No Cq	44.4a	29.0
	1.00E−08	39.2	No Cq	No Cq	No Cq	29.0
	1.00E−09	No Cq	No Cq	No Cq	No Cq	29.1
ASFV	1.00E−01	23.5	No Cq	No Cq	23.9	28.2
	1.00E−02	26.8	No Cq	No Cq	27.3	28.5
	1.00E−03	30.2	No Cq	No Cq	30.6	28.8
	1.00E−04	34.1	No Cq	No Cq	34.3	28.6
	1.00E−05	36.7	No Cq	No Cq	38.5	28.6
	1.00E−06	38.8	No Cq	No Cq	No Cq	28.9
	1.00E−07	No Cq	No Cq	No Cq	No Cq	29.0
SuHV-1	1.00E−03	19.6	No Cq	No Cq	19.8	28.3
	1.00E−04	22.9	No Cq	No Cq	23.0	28.4
	1.00E−05	26.2	No Cq	No Cq	26.5	29.0
	1.00E−06	29.4	No Cq	No Cq	29.6	28.9
	1.00E−07	33.2	No Cq	No Cq	33.1	28.8
	1.00E−08	36.0	No Cq	No Cq	36.6	29.0
	1.00E−09	39.3	No Cq	No Cq	42.6	29.1
FMDV	1.00E−04	25.5	No Cq	No Cq	28.7	28.5
	1.00E−05	28.5	No Cq	No Cq	31.3	28.5
	1.00E−06	31.4	No Cq	No Cq	35.3	28.4
	1.00E−07	34.4	No Cq	No Cq	No Cq	28.3
	1.00E−08	No Cq	No Cq	No Cq	No Cq	28.3

^aOnly one of the duplicates scored positive.