Table 3.
Results of the multiplex real-time PCR assay for mixtures of genomic DNA from MRCoNS and MSSA carrying SCCmec remnants
| DNA samplea | Proportion |
CT difference between SCCmec-orfX and mecA (CTSCC)b | CT difference between 16S rRNA and mecA (CT16S)b | |
|---|---|---|---|---|
| MRCoNS (%) | MSSA (%) | |||
| A | 95 | 5 | 15.99 ± 1.48 (13.03 ∼ 16.94) | 0.87 ± 0.50 (0.20 ∼ 1.34) |
| B | 90 | 10 | 14.60 ± 1.52 (11.75 ∼ 16.15) | 0.74 ± 0.17 (0.51 ∼ 0.92) |
| C | 80 | 20 | 13.15 ± 1.74 (10.33 ∼ 15.34) | 0.90 ± 0.44 (0.36 ∼ 1.38) |
| D | 70 | 30 | 12.32 ± 1.96 (9.36 ∼ 15.00) | 0.59 ± 0.23 (0.19 ∼ 0.85) |
| E | 60 | 40 | 11.54 ± 1.79 (8.79 ∼ 14.29) | 0.45 ± 0.51 (−0.13 ∼ 1.10) |
| F | 50 | 50 | 12.25 ± 4.42 (8.34 ∼ 20.75) | 0.46 ± 0.62 (−0.65 ∼ 1.01) |
| G | 40 | 60 | 10.41 ± 2.41 (7.38 ∼ 14.59) | 0.27 ± 0.22 (−0.01 ∼ 0.57) |
| H | 30 | 70 | 9.25 ± 2.23 (6.46 ∼ 12.43) | −0.19 ± 0.40 (−0.72 ∼ 0.22) |
| I | 20 | 80 | 7.81 ± 2.00 (4.94 ∼ 9.71) | −0.56 ± 0.91 (−1.85 ∼ 0.56) |
| J | 10 | 90 | 6.91 ± 1.64 (4.85 ∼ 8.59) | −1.55 ± 1.00 (−2.81 ∼ −0.47) |
| K | 5 | 95 | 5.66 ± 1.34 (3.84 ∼ 7.01) | −2.06 ± 0.69 (−2.99 ∼ −0.95) |
a
Six samples each of 11 types of DNA were used.
b
The threshold cycle values represent ranges of means ± standard deviations.