Table 1.

Oligonucleotide primers used for amplification and sequencing of complete coding sequences

Primer	Sequence (5′ to 3′)	Polarity	Amplicon size (bp)	Position (bp)	Annealing temp (°C)	Use of primera
atpA JunF1	TTGTTAAAATTAAAAGCAGAAGAAATTAGCG	Forward	1,512	1–31	54	A, S
atpA JunR1	CTAGAGAGTTAGACTTAGTTTAAATTCTTCAATCGC	Reverse		1477–1512		A, S
ATPA-F	CCCCCCTATTCTCCCTTGCCC	Forward		1270–1290		S
ATPA-R	CAAGCCCCTATCGCCGGTATC	Reverse		169–189		S
AtpA_fw_in	TACCCGTAGGCGATGCGGTC	Forward		293–312		S
efp JunF1	ATGGCCATTGGAATGGGTGAAC	Forward	567	1–22	53	A, S
efp JunR1	TTAAACTTTGAGCTTTTCTAAATACTCC	Reverse		540–567		A, S
EFP-F	GCCTAATTTTAGAGGGGATAC	Forward		414–434		S
EFP-R bis	ATTAGGCTCTTCGCACTTATCC	Reverse		174–195		S
mutY JunF1	ATGTCCCAGCAAACCCAAATTGAGGTTG	Forward	873	1–28	58	A, S
mutY JunR1	CTACAAAATCCCCGTCTTTTCTAGTAATACC	Reverse		843–873		A, S
MUTY-F	AGTACCGCGTGCAAGTTTGGC	Forward		719–739		S
MUTY-R	CACGGGCATAATAACCTAGAC	Reverse		125–145		S
ppa JunF1	ATGAACATCTCAAAAATTCCAGTTAGTAACGC	Forward	528	1–32	56	A, S
ppa JunR1	TCAACCTTGGGCTTGTTGGTAATTTGCAAGTGC	Reverse		496–528		A, S
PPA-F2	CATTGTGCTACAAAAGATCAGAC	Forward		378–400		S
PPA-R2	TAGCTGGATATACCATAGATG	Reverse		134–154		S
trpC JunF1	ATGCATGATTTTTTAACAACCATGTTAGAA C	Forward	1,359	1–31	57	A, S
trpC JunR1	TCAATACTCCCGTAACATTTTTGCAATTTGTGC	Reverse		1327–1359		A, S
TRPC-F	GCTAGCTGGAGGGTTAAATGC	Forward		1209–1229		S
TRPC-R	GAGGGGGAGGCTTGTTTGCAC	Reverse		150–170		S
TRPC_rv_in	TTTAAGCGCAGTTGGCATGCA	Reverse		960–980		S
ureI JunF1	ATGCTAGGACTTGTGTTATTGTATGTTGCGATCG	Forward	588	1–34	55	A, S
ureI JunR1	TTACACCCAGTGTTCGATAAAGAGAAGCCAAGCAGG	Reverse		553–588		A, S
UREI-F	ACTGGTTGGATTGAGGAAGTGG	Forward		463–484		S
UREI-R	CAAACAATAGAGATCCCACCTAC	Reverse		106–128		S
yphC JunF1	ATGCTTAAAATTGCTATTTTGGGCAAGCC	Forward	1,317	1–29	57	A, S
yphC JunR1	TCATGTTTGCAAAGTTTCTTCTTTGCCTCTGGC	Reverse		1285–1317		A, S
YPHC-F2	TTGCTTTGGTTATGAATCGCCCTAAG	Forward		1175–1200		S
YPHC-R	AAAGCTCCACCCCATGGCCTGCTAG	Reverse		136–160		S

^aA, primer used for amplification; S, primer used for sequencing.