Fig 5.

vSP-1 interacts with RTA at the PARS domain. (A) vSP-1 complexes with RTA protein. 293T cells were cotransfected with an RTA expression vector and a Flag-vSP-1 or empty (E) vector, followed by immunoprecipitation (IP) with an anti-Flag antibody and Western blotting for RTA. WCE, whole-cell extracts. (B) Mapping of RTA for the vSP-1 binding region. (Top) A set of deletion mutants of pCR3.1-ORF50 was constructed. The positions of deleted amino acids for each construct are given on the maps. (Bottom) The deletion mutants were used for coimmunoprecipitation as described in the legend to panel A. WT, wild type.